New saliva test can rapidly identify paracetamol overdose

Taking a saliva test from a woman's mouth with cotton swab

A research team led by the University of Liverpool has developed an innovative new technique that can rapidly assess paracetamol levels from saliva.

The research was supported by the Engineering and Physical Sciences Research Council (EPSRC) and published in the journal BMC Medicine.

The team demonstrates the clinical validity of a new mass spectrometry technology-based technique that can test a tiny drop of saliva on a piece of paper for levels of paracetamol.

Called paper-arrow mass spectrometry (PA-MS), the patent pending technique was found to offer enhanced analytical performance while requiring less time, resources and clinical involvement than current clinical tests.

Misuse, overdose and severe health risks

Paracetamol is the most consumed medicine globally and its accessibility contributes to frequent misuse and overdose.

Approximately 100,000 cases of paracetamol overdose are reported in the UK each year, leading to 50,000 hospital admissions due to liver toxicity.

If not treated quickly, paracetamol overdoses can lead to severe outcomes, including liver failure, transplantation or even death.

N-acetylcysteine is a medication that is used to treat paracetamol overdose and is effective in preventing acute liver injury.

However, it is time sensitive and most effective if administered within eight hours.

The research team tested the technique using both saliva and plasma samples from volunteers who had ingested paracetamol, and saliva was found to be the preferred sample method.

The study is in collaboration with Alder Hey Children’s Hospital, Royal Liverpool University Hospital, and Liverpool School of Tropical Medicine.

A non-invasive and cost-effective solution

Dr Robert Felstead, Deputy Director of Healthcare Technologies at EPSRC said:

This research, which was supported by EPSRC, represents a significant breakthrough in the rapid and accurate quantitation of paracetamol.

Given the high incidence of paracetamol overdose and its severe consequences, this innovative technology offers a fast, non-invasive and cost-effective solution that can greatly enhance patient care and outcomes.

Dr Simon Maher, an expert in mass spectrometry technologies with the University of Liverpool’s Department of Electrical Engineering and Electronics, said:

This study demonstrates successful testing of the new PA-MS test in a clinical environment and lays the foundation for advancing point-of-care testing across emergency and routine clinical settings.

This innovation represents a significant step forward in ambient ionisation mass spectrometry techniques, with the potential for broad application in clinical diagnostics.

Providing faster diagnosis

Professor Dan Hawcutt, a clinical pharmacologist and Director of Research at Alder Hey Children’s Hospital, and Director of the NIHR Alder Hey Clinical Research Facility, said:

The speed, simplicity, and efficiency of this new technology has the potential to provide faster diagnosis, better patient outcomes, and cost savings for healthcare systems.

Top image:  Credit: AndreyPopov, iStock, Getty Images Plus via Getty Images

This is the website for UKRI: our seven research councils, Research England and Innovate UK. Let us know if you have feedback or would like to help improve our online products and services.